Cellular and Molecular Metabolism Core

The goal of the Cell and Molecular Metabolism Core is to provide support and resources for metabolic phenotyping and mechanistic studies though animal and cell-based approaches to advance understanding of metabolic pathways contributing to health and disease.

Cellular metabolism is central to conveying environmental cues, providing fuel sources, and maintaining energy balance. Disruption or imbalances in cell metabolism impinge upon cellular function and underlie cellular stress associated with obesity and nutrient-related disease pathologies.

- Insulin tolerance test
- Glucose tolerance test
- Hyperinsulinemic-euglycemic glucose clamp studies (Contact the Core for additional tracer studies)
- Catheters and IV/IP injections
- Triglycerides, free fatty acids, cholesterol, glycerol, bile acids
- Cholesterol and triglyceride profile (FPLC)
- In vivo triglyceride secretion and clearance
- In vivo lipogenesis
- 26 cage temperature and humidity-controlled indirect calorimetry system
- Energy expenditure
- Fuel utilization
- Locomotor activity
- Continuous measures of food and water intake
- Continuous body temperature and glucose monitoring
  Running wheel activity
- Statistical analysis support using CalR (PMID: 40993210)
- Noninvasive body composition analysis for measures of fat mass, lean body mass and water content in conscious mice
- Biopsy sample triglyceride content in tissue samples as small as 30mg
- Tissue and blood collection for subsequent processing
- Urine/feces collection
- Core body temperature
- Temperature controlled chambers (4°C – 34°C) for cold or heat challenges
• Seahorse XFe96 for real-time, label-free measurement of cellular metabolism
• Primary preadipocyte and hepatocyte preparations from mice
- Insulin, leptin, adiponectin
- For additional assays, contact the Core.
- Targeted and unbiased
- In vitro and in vivo tracers

Services

The Core will provide HNORC members with access to technology and services for mouse metabolic phenotyping, for comprehensive analysis of cellular respiration, metabolomics, and metabolite tracing, and to support in vitro genetic studies.

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